|
| Status |
Public on Jan 26, 2013 |
| Title |
sip53 TNF [MO157_HuST] |
| Sample type |
RNA |
| |
|
| Source name |
PANC1, sip53, TNFa (0.5 ng/ml, 24 hrs)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Epithelial cancer cell
cell line: PANC1
|
| Treatment protocol |
0.5 ng/ ml of TNFa was added and 24 hours later cells were harvested
|
| Growth protocol |
PANC1 cells were were maintained at 37°C in a standard 5% CO2 humidified incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with a NucleoSpin RNA II kit (Macherey-Nagel).
|
| Label |
biotin
|
| Label protocol |
RNA ( 10μg) was labeled and hybridized to Affymetrix GeneChip Human Exon 1.0 ST arrays
|
| |
|
| Hybridization protocol |
According to Affymetrix standard protocols
|
| Scan protocol |
According to Affymetrix standard protocols, using Affymetrix GeneChip 3000 7G plus scanner
|
| Data processing |
RMA was carried out using Partek Genomic Suite 6.5 (Inc. St. Charles, MO; www.partek.com)
|
| |
|
| Submission date |
Jan 24, 2013 |
| Last update date |
Jan 26, 2013 |
| Contact name |
Tomer Cooks |
| E-mail(s) |
[email protected]
|
| Organization name |
WIS
|
| Street address |
250 Herzel
|
| City |
Rehovot |
| ZIP/Postal code |
67100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE43738 |
Expression data from inflamed PANC1 cells |
|
