|
| Status |
Public on Aug 01, 2014 |
| Title |
Adjacent normal tissue from patient 249 |
| Sample type |
RNA |
| |
|
| Source name |
Adjacent normal tissue from patient 249
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: Adjacent normal tissue
patient: patient 249
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the specimens using Trizol reagent (Invitrogen) and purified with mirVana miRNA Isolation Kit (Ambion, Austin, TX, USA). RNA purity and concentration were determined from OD260/280 readings using spectrophotometer (NanoDrop ND-1000) and RNA integrity was determined by 1% formaldehyde denaturing gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
The extracted RNA were labeled according to the Agilent manufacturer's instructions. Briefly, the miRNAs were labeled using the Agilent miRNA labeling reagent. Total RNA (100 ng) was dephosphorylated and ligated with pCp-Cy3.
|
| |
|
| Hybridization protocol |
The labeled RNA was purified and hybridized to miRNA arrays according to the Agilent manufacturer's instructions.
|
| Scan protocol |
Images were scanned with the Agilent microarray scanner (Agilent), gridded, and analyzed using Agilent feature extraction software version 10.10.
|
| Description |
Adjacent normal tissue from patient 249
|
| Data processing |
The miRNA array data were analyzed for data summarization, normalization and quality control by using the Percentile Shift (90th percentile) with GeneSpring software V11.5 (Agilent).
|
| |
|
| Submission date |
Jan 24, 2013 |
| Last update date |
Aug 01, 2014 |
| Contact name |
Jiagen Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Cancer Institute, CAMS
|
| Street address |
17 Panjiayuannanli Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100021 |
| Country |
China |
| |
|
| Platform ID |
GPL16543 |
| Series (1) |
| GSE43732 |
MiRNA Expression Profile Study Reveals a Prognostic Signature for Esophageal Squamous Cell Carcinoma |
|
