|
| Status |
Public on Mar 01, 2013 |
| Title |
MCF7, 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen + 20 nM endoxifen, 24 h, rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Parental MCF7 cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: 10 nM estradiol + 300 nM tamoxifen + 7 nM 4HT + 700 nM N-desmethyl-tamoxifen + 20 nM endoxifen
cell line: MCF7
cell type: breast cancer
|
| Treatment protocol |
One day prior to and during ligand treatments, cells were cultured in the same media containing 10% 3X charcoal stripped FBS to deplete them of steroid exposure.
|
| Growth protocol |
MCF7 cells were routinely cultured in phenol red-free Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12) medium containing 10% (v/v) Fetal Bovine Serum (FBS) and 1% (v/v) antibiotic-antimycotic (AA) solution in a humidified 37°C incubator with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol reagent.
|
| Label |
Cy3
|
| Label protocol |
standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 3
Sample name: 75
|
| Data processing |
The raw data was pre-processed by Illumina's beadstudio without backgroud subtraction and probe level profile was obtained. This data was exported, log2 ransformed and normalized using fast lowess normalization in R
|
| |
|
| Submission date |
Jan 23, 2013 |
| Last update date |
Mar 18, 2013 |
| Contact name |
John Hawse |
| E-mail(s) |
[email protected]
|
| Phone |
507-284-4268
|
| Organization name |
Mayo Clinic
|
| Department |
Biochemistry and Molecular Biology
|
| Street address |
200 1st St. SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE43702 |
Gene expression profiles of MCF7 breast cancer cells following estrogen and anti-estrogen treatment. |
|
