|
| Status |
Public on Jan 17, 2013 |
| Title |
Hypothalamus from HF-fed control rats |
| Sample type |
RNA |
| |
|
| Source name |
Rat hypothalamus
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
age: 4 months
tissue: Hypothalamus
diet: HF-fed
rats: control rats
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the TargetAmp Nano-g Biotin-aRNA Labeling for Illumina System
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Hyp control HF
|
| Data processing |
The data were normalised using cubic spline normalisation with GenomeStudio (Illumina)
|
| |
|
| Submission date |
Jan 16, 2013 |
| Last update date |
Jan 17, 2013 |
| Contact name |
christophe breton |
| E-mail(s) |
[email protected]
|
| Phone |
+33(0)320436532
|
| Organization name |
University of Lille 1
|
| Street address |
Cité Scientifique
|
| City |
Villeneuve d'Ascq |
| ZIP/Postal code |
59650 |
| Country |
France |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE43547 |
Genome-wide analysis of hypothalamic gene expression in prenatally undernourished male rat offspring under high fat diet |
|
