|
| Status |
Public on Feb 28, 2013 |
| Title |
Hif-2αF/F |
| Sample type |
RNA |
| |
|
| Source name |
Colon
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: colon
genotype/variation: Hif-2αF/F
age: 6 to 8 weeks old
|
| Treatment protocol |
Colon mucosal scrapings were got from 6 to 8 weeks old mice treated with 3% DSS for 3 days
|
| Growth protocol |
All mice were C57BL/6 background and maintained in standard cages in a light and temperature-controlled room and were allowed standard chow and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA were prepared according to the Ambion Premier kit protocol from 250 ng total RNA (MessageAmp Premier RNA Amplification Kit Instruction Manual, 2008, Ambion).
|
| |
|
| Hybridization protocol |
Following fragmentation, 16 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
|
| Description |
Gene expression data from colons.
|
| Data processing |
Data was analyzed using the affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
|
| |
|
| Submission date |
Jan 10, 2013 |
| Last update date |
Feb 28, 2013 |
| Contact name |
XIANG XUE |
| E-mail(s) |
[email protected]
|
| Phone |
7346155041
|
| Organization name |
Umich
|
| Street address |
1301 E. Catherine Street
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE43416 |
Gene Expression profiles of colon from Hif-2αF/F, Hif-2αlΔIE treated with DSS |
|
