|
| Status |
Public on Dec 03, 2018 |
| Title |
brain mnr rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
brain mnr
|
| Organism |
Papio hamadryas |
| Characteristics |
gender: female
developmental stage: 165 dGa
tissue: brain (frontal cortex)
mother's pregnancy condition: nutrient restricted
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
IL_MNR_8
replicate 2
|
| Data processing |
The data were background corrected with GenomeStudio v1.9.0. Data was customized to include only probes that were detected at greater than or equal to 95% confidence in all baboon samples of at least one tissue type from previous Illumina gene expression experiments (n=22,148). These included probe signals from fetal baboon brain (n=12), liver (n=12), placenta (n=12), and kidney (n=12). All median normalisation and log2 transformation was performed on the filtered dataset in Genesifter Analysis Edition (http://www.geospiza.com/Products/AnalysisEdition.shtml).
|
| |
|
| Submission date |
Dec 05, 2012 |
| Last update date |
Dec 03, 2018 |
| Contact name |
Laura L Cox |
| Organization name |
Texas Biomedical Research Institute
|
| Department |
Genetics
|
| Lab |
Cox Lab
|
| Street address |
7620 NW Loop 410
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78227-5301 |
| Country |
USA |
| |
|
| Platform ID |
GPL6097 |
| Series (1) |
| GSE42756 |
Effects of Intrauterine Growth Restriction on Ribosomal Subunit mRNA and Protein Expression in the Frontal Cortex of Near-Term Fetal Baboons |
|
