|
| Status |
Public on Aug 01, 2013 |
| Title |
5-LSK_Tet2_Ezh2_DKO_MDS/MPN |
| Sample type |
RNA |
| |
|
| Source name |
Tet2KD/KDEzh2-/- MDS/MPN LSK from BM
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: LSK
cells purified from: BM
genotype/variation: Tet2KD/KDEzh2-/- MDS/MPN
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
250 ng of total RNA was mixed with spike-in controls using an Agilent One Color Spike Mix Kit (Agilent Technologies), amplified and labeled with Cyanine 3 using a Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer's instructions, which generated single-color labeled cRNA.
|
| |
|
| Hybridization protocol |
1,650 ng of the labeled cRNA was hybridized to Agilent Whole Mouse Genome 4x44k array Ver.2, Design ID: 028005. The process of hybridization and washing was performed using a Hi-RPM Gene Expression Hybridization Kit (Large) (Agilent Technologies) and a Gene Expression Wash Pack (Agilent Technologies) respectively.
|
| Scan protocol |
GeneChips were scanned using a DNA microarray scanner (Agilent Technologies)
|
| Description |
Gene expression data of LSK from Tet2KD/KDEzh2-/- mice that developed myelodysplastic/myeloproliferative neoplasm (MDS/MPN)
|
| Data processing |
standard Agilent protocol
|
| |
|
| Submission date |
Dec 02, 2012 |
| Last update date |
Aug 01, 2013 |
| Contact name |
ATSUSHI IWAMA |
| E-mail(s) |
[email protected]
|
| Phone |
81-43-226-2187
|
| Fax |
81-43-226-2191
|
| Organization name |
Graduate School of Medicine, Chiba University
|
| Department |
Department of Cellular and Molecular Medicine
|
| Street address |
1-8-1 Inohana, Chuo-ku
|
| City |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE42666 |
Expression data from Tet2-hypomorph (knockdown) and/or Ezh2-null Lineage-Sca-1+c-Kit+ (LSK) cells and granulocyte-macrophage progenitors (GMPs) |
|
