|
| Status |
Public on Dec 01, 2013 |
| Title |
Z138 2.5uM AEB071 3h rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Z-138 cells
|
| Organism |
Homo sapiens |
| Characteristics |
mantle cell lymphoma (mcl) line: Z-138
stn sensitivity: insensitive
agent: 2.5uM Sotrastaurin
|
| Treatment protocol |
3 million cells were resuspended in 3ml of fresh media in 6 well dishes. The next day, DMSO or Sotrastaurin was added for 3hrs.
|
| Growth protocol |
Jeko-1 and Mino cells were cultured in 20% FBS RPMI; Z-138 and Maver-1 cells were cultured in 10% FBS RPMI.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plus kit (qiagen) following the manufacturers instructions
|
| Label |
biotin
|
| Label protocol |
Nugen Ovation RNA Amplification System+ WB + FL cDNA Biotin Module V2 (NuGen Lts)
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip eukaryotic hybridization protocol (Affymetrix Inc.)
|
| Scan protocol |
GeneChip Scanner 3000 (Affymetrix Inc.)
|
| Description |
Gene expression data from Z-138 cells treated with 2.5uM Sotrastaurin
|
| Data processing |
Micorarray Analysis Suite version 5.0 (MAS 5.0) background correction, scaled to 150
|
| |
|
| Submission date |
Nov 27, 2012 |
| Last update date |
Dec 01, 2013 |
| Contact name |
Joshua M Korn |
| E-mail(s) |
[email protected]
|
| Organization name |
Novartis
|
| Street address |
250 Mass Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02141 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE42549 |
Transcriptional responses of mantle cell lymphoma (MCL) lines to PKC inhibition |
|
