|
| Status |
Public on May 26, 2013 |
| Title |
HSC1_CB1 |
| Sample type |
RNA |
| |
|
| Source name |
human cord blood progenitor cells
|
| Organism |
Homo sapiens |
| Characteristics |
sorted cell type: hematopoietic stem cells (HSC1)
cell surface expression: Lin- CD34+ CD38- CD45RA- CD90+ CD49f+
|
| Treatment protocol |
Lineage-depleted cord blood cells were thawed and flow-sorted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from 2,000-5,000 sorted cells with Trizol reagent.
|
| Label |
biotin
|
| Label protocol |
Biotin.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Scanned on a BeadArray™ Reader using BeadScan software (v3.2).
|
| Data processing |
The data were normalized using quantile normalization in R (v2.12.1).
|
| |
|
| Submission date |
Nov 20, 2012 |
| Last update date |
May 26, 2013 |
| Contact name |
Elisa Laurenti |
| Organization name |
University of Cambridge
|
| Department |
Haematology
|
| Lab |
Laurenti laboratory
|
| Street address |
Jeffrey Cheah Biomedical Centre
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL14951 |
| Series (1) |
| GSE42414 |
The transcriptional architecture of early human hematopoiesis identifies multilevel control of lymphoid commitment. |
|
