|
| Status |
Public on Dec 01, 2012 |
| Title |
parthenogenetic stem cell line pES3 |
| Sample type |
RNA |
| |
|
| Source name |
pES3
|
| Organism |
Homo sapiens |
| Characteristics |
source: embryonic
cell type: parthenogenetic stem cell line
cell line: pES3
|
| Treatment protocol |
nuclear genome swere exchanged between oocytes of two different donors, thereby exchanging the mitochondrial genotype. Oocytes were activated, allowed to develop to the blastocyst stage, and embryonic stem cell lines were derived.
|
| Growth protocol |
human embryonic stem cells cultured in knockout serum containing medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy Plus Mini kit
|
| Label |
biotin
|
| Label protocol |
Biotin label introduced according to protocols of the Illumina Total Prep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Hybridization according to Illumina protocols on Illumina HumanHT-12 v4 Expression BeadChip. Arrays are hybridized, washed, blocked and stained with streptavidin-Cy3. The protocol is WGGEX Direct Hybridization Assay Guide (11322355 A).
|
| Scan protocol |
According to Illumina protocols with the BeadArray Reader. Protocols can be found on the illumina web page.
|
| Description |
1 of 1 samples
6004838015
B
SAMPLE 7
|
| Data processing |
Data were normalized to average signal. Background was subtracted.
|
| |
|
| Submission date |
Nov 06, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Dieter Egli |
| E-mail(s) |
[email protected]
|
| Phone |
617 4968659
|
| Organization name |
The New York Stem Cell Foundation Research Institute
|
| Department |
NYSCF
|
| Lab |
Egli
|
| Street address |
3960 Broadway
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL14951 |
| Series (1) |
| GSE42077 |
Elimination of mitochondrial DNA variants by nuclear genome transfer in human oocytes |
|
