|
| Status |
Public on Oct 30, 2012 |
| Title |
keratinocytes_anti-miR-Ctrl_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
keratinocytes transfected with anti-miR-Ctrl
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: skin
cell type: keratinocytes
hairpin rna: anti-miR-Ctrl
|
| Treatment protocol |
The third passage keratinocytes at 30-50% confluence were transfected with 10 nM miR-31 hairpin inhibitor or microRNA hairpin inhibitor negative control #1 (ThermoFisher Scientific) using Lipofectamine 2000 (Invitrogen).
|
| Growth protocol |
Human adult epidermal keratinocytes were purchased from Cascade Biologics and cultured in EpiLife® serum-free keratinocyte growth medium including Human Keratinocyte Growth Supplement at a final Ca2+ concentration of 0.06mM (Cascade Biologics) at 37°C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
48 hours after transfection, the total RNA was extracted with the miRNeasy Mini kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled using the Whole Transcript (WT) Sense Target Labeling Assay according to the manufacturer's (Affymetrix) instructions.
|
| |
|
| Hybridization protocol |
The samples were hybridized to a Human Gene 1.1 ST Array Plate according to the standard Affymetrix GeneTitan protocol.
|
| Scan protocol |
The array plate was scanned using the Affymetrix GeneTitan Scanner.
|
| Description |
Anti-miR-Ctrlb
Gene expression data from keratinocytes transfected with control RNA with scrambled sequence.
|
| Data processing |
Generated CEL files were analyzed using Affymetrix Expression Console v. 1.1 (Summarization: Plier, Background Correction: PM-GCBG, Normalization: Global Median).
|
| |
|
| Submission date |
Oct 29, 2012 |
| Last update date |
Oct 30, 2012 |
| Contact name |
Ning Xu |
| Organization name |
Karolinska Institutet
|
| Street address |
CMM L8:02
|
| City |
Stockholm |
| ZIP/Postal code |
17176 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE41905 |
A global transcriptome analysis of keratinocytes upon suppression of endogenous microRNA-31 |
|
