|
| Status |
Public on Mar 04, 2013 |
| Title |
Normal_WT_NPOOL_MP |
| Sample type |
RNA |
| |
|
| Source name |
Pooled normal mammary tissue
|
| Organism |
Mus musculus |
| Characteristics |
gender: Female
strain: FVB
tissue: Normal
genotype: Wild-Type
parity: Multiparous
pathology: NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from mouse mammary tumors and normal mammary glands that had been snap-frozen immediately following animal necropsy. Tissues were powdered under liquid nitrogen, homogenised in Qiashredder columns and RNA isolated using the miRNeasy Mini Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Labeling was carried out with the miRNA Complete Labeling and Hyb Kit and microRNA Spike-in kit (both Agilent).
|
| |
|
| Hybridization protocol |
miRNA was hybridized to Agilent 8x60K miRNA Oligo Microarrays (G4872A-038112). Arrays were washed as directed by the manufacturer.
|
| Scan protocol |
Agilent dual laser scanner (P/N: G2505B). Feature extraction was carried out using Agilent software (FE 10.7.3).
|
| Data processing |
Raw Cy3 intensities were background corrected using the RMA background correction algorithm. Data was log2 transformed and quantile normalized.
|
| |
|
| Submission date |
Oct 19, 2012 |
| Last update date |
Mar 05, 2013 |
| Contact name |
Robert Lesurf |
| Organization name |
Washington University in St. Louis
|
| Department |
McDonnell Genome Institute
|
| Lab |
Griffith Lab
|
| Street address |
4444 Forest Park Ave
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63112 |
| Country |
USA |
| |
|
| Platform ID |
GPL16199 |
| Series (2) |
| GSE41723 |
Met synergizes with p53 loss to induce mammary tumors that possess features of claudin-low breast cancer (miRNA expression data). |
| GSE41748 |
Met synergizes with p53 loss to induce mammary tumors that possess features of claudin-low breast cancer |
|
