|
| Status |
Public on Oct 31, 2012 |
| Title |
Non-Retinoblastoma serum -Replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
Whole blood serum
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Non-Retinoblastoma
|
| Treatment protocol |
Freshly isolated whole blood serum from normal healthy individual and Retinoblastoma patients were obtained then perserved at -80 ºC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the mirVana RNA Isolation kit(Ambion) following the manufacturer's recommendations
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared using miRNA Complete Labeling and Hyb Kit(Agilent) according to the manufacturer's instructions
|
| |
|
| Hybridization protocol |
The hybridization used miRNA Complete Labeling and Hyb Kit(Agilent) according to the manufacturer's instructions
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent Microarray Scanner
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.0 (Agilent) using default parameters
|
| |
|
| Submission date |
Oct 03, 2012 |
| Last update date |
Oct 31, 2012 |
| Contact name |
madavan vasudevan |
| E-mail(s) |
[email protected]
|
| Phone |
+91-9845093355
|
| Organization name |
Theomics International Private Limited
|
| Street address |
Kasturi Nagar
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560043 |
| Country |
India |
| |
|
| Platform ID |
GPL14767 |
| Series (1) |
| GSE41321 |
Differential microRNA expression profiles in Retinoblastoma serum |
|
