|
| Status |
Public on Nov 27, 2012 |
| Title |
Ex 3-4 DSC3 3 |
| Sample type |
RNA |
| |
|
| Source name |
SK-N-AS ATCC Nr. CRL-2137
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SK-N-AS (ATCC® CRL-2137)
gender: female
age: 6
ethnicity: caucasian
tissue: brain
disease: neuroblastoma
cell type: neuroblast
derived from metastatic site: bone marrow
transfection: cells transfected with pcDNA 3.1 exon d3-d4/DSC3
|
| Growth protocol |
Cells were grown in DMEM (10% FCS; 37°C; 5% CO2) containing geniticin (400 µg/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were scratched from surface, peletted and total RNA was extracted using RNeasy Kit, Qiagen.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 21
|
| Data processing |
The data were normalised using quantile normalisation in R
|
| |
|
| Submission date |
Sep 26, 2012 |
| Last update date |
Dec 19, 2012 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
[email protected]
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE41157 |
Disease-specific sequence change DSC3 in TAF1/DYT3 affects genes in vesicular transport and dopamine metabolism |
|
