|
| Status |
Public on Jun 01, 2006 |
| Title |
CD4+ splenocytes at 6h, biological replicate 1 sham-5 |
| Sample type |
RNA |
| |
|
| Source name |
negatively selected CD4+ splenocytes from an individual mouse treated with sham laparotomy
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: C57Bl/6 mice
Age: 8-12 weeks old
|
| Treatment protocol |
Each mouse was anesthetized under halothane, its cecum exteriorized by laparotomy and manipulated (sham). The cecum was then replaced and the belly closed in two layers. Six hours later the spleen was harvested and the mouse euthanized. The spleen was disaggregated over nylon mesh and CD4+ splenocytes were enriched by immunomagnetic selection (Miltenyi) according to manufacturer's instructions, lysed and RNA isolated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted according to the manufacturer's instructions (RNeasy, Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using a two round amplification strategy for small (<1 microgram) samples according to the manufacturer's instructions (Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on the GeneChip Mouse Transcriptome 430 2.0 (Affymetrix) according to the manufacturer's instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner G3000.
|
| Description |
Gene expression data from CD4+ splenocytes from a sham-manipulated mouse 6h after the induction of sham laparotomy.
|
| Data processing |
The data were analyzed with dChip.
|
| |
|
| Submission date |
Mar 15, 2006 |
| Last update date |
Mar 15, 2006 |
| Contact name |
Jonathan E McDunn |
| E-mail(s) |
[email protected]
|
| Phone |
314-747-0054
|
| Fax |
314-362-7474
|
| Organization name |
Washington University School of Medicine
|
| Department |
Surgery
|
| Street address |
660 South Euclid Avenue
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE4479 |
Expression data from CD4+ splenocytes 6h post-sepsis |
|
