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Sample GSM100794 Query DataSets for GSM100794
Status Public on Jun 01, 2006
Title septic CD4+ splenocytes at 6h, biological replicate 2
Sample type RNA
 
Source name negatively selected CD4+ splenocytes from an individual mouse treated with cecal ligation and puncture
Organism Mus musculus
Characteristics Genotype: C57Bl/6 mice
Age: 8-12 weeks old
Treatment protocol Each mouse was anesthetized under halothane, its cecum exteriorized by laparotomy and ligated and punctured (septic). The cecum was then replaced and the belly closed in two layers. Six hours later the spleen was harvested and the mouse euthanized. The spleen was disaggregated over nylon mesh and CD4+ splenocytes were enriched by immunomagnetic selection (Miltenyi) according to manufacturer's instructions, lysed and RNA isolated.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted according to the manufacturer's instructions (RNeasy, Qiagen)
Label biotin
Label protocol Biotinylated cRNA was prepared using a two round amplification strategy for small (<1 microgram) samples according to the manufacturer's instructions (Affymetrix)
 
Hybridization protocol Following fragmentation, cRNA was hybridized on the GeneChip Mouse Transcriptome 430 2.0 (Affymetrix) according to the manufacturer's instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner G3000.
Description Gene expression data from CD4+ splenocytes from a septic mouse.
Data processing The data were analyzed with dChip.
 
Submission date Mar 15, 2006
Last update date Mar 15, 2006
Contact name Jonathan E McDunn
E-mail(s) [email protected]
Phone 314-747-0054
Fax 314-362-7474
Organization name Washington University School of Medicine
Department Surgery
Street address 660 South Euclid Avenue
City Saint Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL1261
Series (1)
GSE4479 Expression data from CD4+ splenocytes 6h post-sepsis

Data table header descriptions
ID_REF Affymetrix Probe-set IDs
VALUE dChip-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 443.902
AFFX-BioB-M_at 535.239
AFFX-BioB-3_at 599.516
AFFX-BioC-5_at 796.451
AFFX-BioC-3_at 1081.66
AFFX-BioDn-5_at 1215.03
AFFX-BioDn-3_at 3607.08
AFFX-CreX-5_at 8026.81
AFFX-CreX-3_at 10720.8
AFFX-DapX-5_at 36.6222
AFFX-DapX-M_at 28.2838
AFFX-DapX-3_at 41.5691
AFFX-LysX-5_at 36.4042
AFFX-LysX-M_at 114.586
AFFX-LysX-3_at 44.849
AFFX-PheX-5_at 37.656
AFFX-PheX-M_at 29.2972
AFFX-PheX-3_at 82.0141
AFFX-ThrX-5_at 56.8745
AFFX-ThrX-M_at 36.178

Total number of rows: 45101

Table truncated, full table size 849 Kbytes.




Supplementary data files not provided

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