array type: Mouse430_2 amount to core: 30 units of amount: ul od260: 2.337 od280: 1.176 ratio: 1.99 dilution: 1:10 rna integrity number: 9.1 bioanalyzer results: excellent sample preservation: RNAlater strain: B6C3F1/Crl ncbi taxonomy id: 10090 individual: 117 developmental state: adult sacrifice method: Pentobarbital (5.2 mg/kg) and exsanguination age: 10 units of age: Weeks at start of exposure tissue: Lung tissue harvest methods: Lung samples were obtained by placing a suture loosely around the left bronchus at the site where the bronchus enters the left lung lobe. A second suture was placed around the right bronchus and pulled tightly to tie off the right lung lobes. The right lung lobes were removed and placed in RNAlater, and the left lung infused under hydrostatic pressure (~30 cm) with neutral buffered formalin. Samples placed in RNAlater were incubated overnight at 4°C and frozen the next day at < 70°C. agent: Chloroprene treatment repeat: 5 units of treatment repeat: days/week treatment duration: 5, Units of Duration: days treatment time: 6 units of treatment time: 6 hr/day vehicle for chemical: Air route of exposure: inhalation dose or concentration: 0.3ppm
Biomaterial provider
L. Pluta, The Hamner Institutes for Health Sciences, 6 Davis Drive, Research Triangle Park, NC, 27709, Russell Thomas, [email protected], 919 558 1311
Treatment protocol
in vivo Treatment: Chloroprene, Treatment Repeat: 5, Units of Treatment Repeat: days/week, Treatment Duration: 5, Units of Duration: days, Treatment Time: 6, Units of Treatment Time: 6 hr/day, Vehicle for Chemical: Air, Route of Exposure: inhalation, Dose or Concentration: 0.3, Units of Dose or Concentration: ppm
RT_03_5d_117_Lung_CLPR-03_RSTSRP805_Mouse430_2_RSTSRP805_1: Female mice (B6C3F1/Crl) were exposed to chloroprene (CAS 126-99-8) by whole-body inhalation exposure at 0, 0.3, 2, 13, or 90 ppm. Exposures were performed for 6 hr/day, 5 days per week. The animals were sacrified at 5 days or 19 days (15 exposure days) post initiation of exposure. The right lung was excised and gene expression microarray analysis was performed using Affymetrix Mouse 430_2 arrays.
