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| Status |
Public on Jun 30, 2017 |
| Title |
Next-generation sequencing facilitates metal homeostasis and intoxication studies |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Analyze transcriptome changes in response to metal treatment in wildtype and sensitized genetic backgrounds.
Methods: Zinc or manganese chloride supplementation of wildtype or mutant Drosophila S2R+ cultured cells, followed by RNA extraction.
Results: Zinc chloride supplementation induces transcriptional responses in wildtype, ia-2 mutant, and ZnT63C mutant cells.
Conclusions: Wildtype S2R+ cells have a transcriptional response to mild zinc chloride supplementation and the zinc response is greater (in degree and in number of genes) in genetically sensitized cell backgrounds that perturb zinc mobilization.
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| Overall design |
The mRNA profiles of wild type or mutant S2R+ cells treated with 1mM ZnCl2 or MnCl2 for 24 hours, or untreated control cells, were generated by deep sequencing, in replicate, using Illumina HiSeq2000.
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| Contributor(s) |
Mohr S, Rudd K, Hu Y, Song W, Perrimon N |
| Citation(s) |
29223976 |
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| Submission date |
May 25, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Yanhui Hu |
| E-mail(s) |
[email protected]
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| Phone |
617-432-6546
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| Organization name |
Harvard Medical School
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| Department |
Genetics
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| Lab |
Norbert Perrimon
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| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (18)
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| GSM2642158 |
WT, Zn treatment rep2 |
| GSM2642159 |
WT, Mn treatment rep1 |
| GSM2642160 |
WT, Mn treatment rep2 |
| GSM2642161 |
ZnT63-KO, no treatment rep1 |
| GSM2642162 |
ZnT63-KO, no treatment rep2 |
| GSM2642163 |
ZnT63-KO, Zn treatment rep1 |
| GSM2642164 |
ZnT63-KO, Zn treatment rep2 |
| GSM2642165 |
ZnT63-KO, Mn treatment rep1 |
| GSM2642166 |
ZnT63-KO, Mn treatment rep2 |
| GSM2642167 |
IA2-KO, no treatment rep1 |
| GSM2642168 |
IA2-KO, no treatment rep2 |
| GSM2642169 |
IA2-KO, Zn treatment rep1 |
| GSM2642170 |
IA2-KO, Zn treatment rep2 |
| GSM2642171 |
IA2-KO, Mn treatment rep1 |
| GSM2642172 |
IA2-KO, Mn treatment rep2 |
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| Relations |
| BioProject |
PRJNA388110 |
| SRA |
SRP108078 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE99332_RAW.tar |
8.8 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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