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| Status |
Public on May 21, 2019 |
| Title |
RNA binding protein DDX1 is responsible for fatty acid mediated repression of insulin translation |
| Organism |
Rattus norvegicus |
| Experiment type |
Other
|
| Summary |
Previous studies have focused on the combined glucolipoxicity that is presence of high glucose and free fatty acid levels effect on insulin gene expression, but the effect of high FFA and normal glucose level on insulin expression is unkown. Here we observed that palmitate can inhibit insulin translation. And we identified a novel insulin mrna binding protein DDX1 that is responsible for fatty acid mediated insulin translation inhibition. Palmitate treatment causes phosphorylation of DDX1 at S295 and dissociation from insulin mRNA, which decreases insulin translation.
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| Overall design |
we used CLIP-seq to identify target genes and the binding sequences of DDX1 in INS1 cell
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| Contributor(s) |
Li Z, Cai Z, Zhong W, Xu T |
| Citation(s) |
30295850 |
| |
| Submission date |
May 21, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Wen Zhong |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Biophysics, Chinese Academy of Sciences
|
| Street address |
Datun Road
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA387363 |
| SRA |
SRP107684 |
