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| Status |
Public on Aug 10, 2017 |
| Title |
A sequence-specific core promoter binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Ribosomal protein (RP) genes must be coordinately expressed for proper assembly of the ribosome yet the mechanisms that control expression of RP genes in metazoans are poorly understood. Recently, TATA-Binding Protein-related factor 2 (TRF2) rather than the TATA-Binding Protein (TBP) was found to function in transcription of RP genes in Drosophila. Unlike TBP, TRF2 lacks sequence-specific DNA binding activity, so the mechanism by which TRF2 is recruited to promoters is unclear. We show that the transcription factor M1BP, which associates with the core promoter region, activates transcription of RP genes. Moreover, M1BP directly interacts with TRF2 to recruit it to the RP gene promoter. High resolution ChIP-exo was used to analyze in vivo the association of M1BP, TRF2, and the TFIID subunit, TAF1. Despite recent work suggesting that TFIID does not associate with RP genes in Drosophila, we find that TAF1 is present at RP gene promoters and that its interaction might also be directed by M1BP. Although M1BP associates with thousands of genes and TRF2 associates with hundreds, their colocalization is largely restricted to RP genes, suggesting that this combination is key to coordinately regulating transcription of the majority of RP genes in Drosophila.
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| Overall design |
ChIP-exo was performed using S2R+ cells. The datasets include replicates for TRF2, TAF1 (TAF250), and Preimmune samples and a single dataset for M1BP.
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| Contributor(s) |
Baumann DG, Gilmour DS |
| Citation(s) |
28977400 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM047477 |
Potentiated State of the HSP70 Heat Shock Gene |
PENNSYLVANIA STATE UNIVERSITY-UNIV PARK |
DAVID Scott GILMOUR |
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| Submission date |
Apr 14, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
David S Gilmour |
| E-mail(s) |
[email protected]
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| Organization name |
Penn State University
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| Street address |
465 North Frear
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| City |
University Park |
| State/province |
Pennsylvania |
| ZIP/Postal code |
16802 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA382999 |
| SRA |
SRP104113 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE97841_RAW.tar |
23.8 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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