|
| Status |
Public on Apr 15, 2017 |
| Title |
Analysis of BRD4-regulated genes in human glioma U251 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
BRD4 is a member of the bromodomain and extra-terminal (BET) family, which has become a promising drug target for numerous cancers. It has been reported that BRD4 is deregulated in gliomas; however, the precise molecular pathways regulated by BRD4 are still elusive.
In this study, we knocked down BRD4 expression in glioma cell line U251 by lentivirus-mediated short hairpin RNA (shRNA) approach. BRD4 downregulation attenuated cell proliferation and promoted cell apoptosis. Genome-wide analysis of BRD4-regulated transcripts in U251 cells was performed using microarray to reveal the possible molecular mechanism
|
| |
|
| Overall design |
To knock down BRD4 expression in U251 cells, lentivirus that expressed shRNA against human BRD4 were transfected U251 cells. Scrambled shRNA (Scr-shRNA) that targeted a non-specific sequence was used as the normal control. The efficiency of BRD4 knockdown were demonstrated by real-time PCR and western blot assays. BRD4-shRNA- transfected and Scr-shRNA-transfected U251 cells were collected for RNA extraction and hybridization on Affymetrix microarrays.
|
| |
|
| Contributor(s) |
Du Z, Liu S, Zhao Y |
| Citation(s) |
29568956 |
| |
| Submission date |
Apr 14, 2017 |
| Last update date |
Oct 16, 2018 |
| Contact name |
Feng jun lin |
| E-mail(s) |
[email protected]
|
| Phone |
15845412541
|
| Organization name |
1Key Laboratory of Cell Differentiation and Apoptosis of Ministry of Education
|
| Department |
Department of Pathophysiology
|
| Lab |
Shanghai Jiao Tong University School of Medicine
|
| Street address |
2Institute of Health Sciences
|
| City |
Shanghai, |
| State/province |
xvhuiqv |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
|
| Samples (6)
|
| GSM2577513 |
U251 cells transfected with BRD4-shRNA, biological rep1 |
| GSM2577514 |
U251 cells transfected with BRD4-shRNA, biological rep2 |
| GSM2577515 |
U251 cells transfected with BRD4-shRNA, biological rep3 |
| GSM2577516 |
U251 cells transfected with Scr-shRNA, biological rep1 |
| GSM2577517 |
U251 cells transfected with Scr-shRNA, biological rep2 |
| GSM2577518 |
U251 cells transfected with Scr-shRNA, biological rep3 |
|
| Relations |
| BioProject |
PRJNA382892 |
Less...
More...