|
| Status |
Public on Nov 28, 2007 |
| Title |
Interferon response of radioresistant and radiosensitive human head&neck tumor cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Nu61, a radiation-resistant human tumor xenograft, was selected from a parental radiosensitive tumor SCC-61 by eight serial cycles of passage in athymic nude mice and in vivo irradiation.
Obtained tumors xenografts were profiled in two independent experiments using Affymetrix U133A arrays. Most abundant gene pattern associated with radioresistant phenotype was presented by IFN-inducible, Stat1-dependent pathway. In these experiments we detected genes responding to IFN alpha, beta and gamma in nu61 and SCC61
Keywords: Pair-wise comparison of un-treated and treated tumor cell lines
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| Overall design |
Cell lines derived from nu61 and SCC61 were grown in vitro and treated by the mixture of IFNalpha, IFNbeta and IFN gamma (100,100 and 5ng/ml respectively). 5 hours after administration of IFNs cells were lysed, RNA collected and used for hybridization with U133A chips to detect genes responding to IFN treatment in nu61 and SCC61
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| Contributor(s) |
Khodarev NN, Beckett M, Labay E, Darga TE, Mauceri HJ, Efimova EV, Pitroda S, Minn AJ, Roizman B, Weichselbaum RR |
| Citation(s) |
14755057, 17909027 |
| |
| Submission date |
Nov 28, 2007 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Nikolai N. Khodarev |
| E-mail(s) |
[email protected]
|
| Phone |
773-834-3282
|
| Fax |
773-702-1968
|
| Organization name |
The University of Chicago
|
| Department |
Radiation and Cellular Oncology
|
| Street address |
5841 South Maryland Avenue
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
|
| Samples (8)
|
| GSM245419 |
Radioresistant tumor cell line-untreated-rep1 |
| GSM245420 |
Radioresistant tumor cell line -untreated-rep2 |
| GSM245421 |
Radioresistant tumor cell line-IFN-treated-rep1 |
| GSM245422 |
Radioresistant tumor cell line -IFN-treated-rep2 |
| GSM245423 |
Radiosensitive tumor cell line-untreated-rep1 |
| GSM245424 |
Radiosensitive tumor cell line -untreated-rep2 |
| GSM245425 |
Radiosensitive tumor cell line-IFN-treated-rep1 |
| GSM245426 |
Radiosensitive tumor cell line -IFN-treated-rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE9716 |
Radioresistant and radiosensitive tumors and cell lines |
|
| Relations |
| BioProject |
PRJNA105097 |
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