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| Status |
Public on Nov 28, 2007 |
| Title |
Identification of putative targets of AP3/PI |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
To identify genes regulated by AP3/PI, we carried out microarray experiments using an Arabidopsis whole genome GeneChip array (ATH1 GeneChip, Affymetrix, Santa Clara, CA) in conjunction with an inducible AP3-GR system. For these experiments, we used 35S::AP3-GR transgenic plants in a 35S::PI, ap3-3 null mutant background for various dex or mock treatments. RNA was extracted from inflorescences at 0 and 4 hours after dex or a mock treatment and used as probes for our microarray experiments. Three biological replicates of each were hybridized to Affymetrix ATH1 arrays. We used the Affymetrix Microarray Suite software (MAS) to identify genes whose expression profiles changed only after dex-treatment and are likely targets of AP3/PI.
Keywords: time course
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| Overall design |
For these experiments, we used 35S::AP3-GR transgenic plants in a 35S::PI, ap3-3 null mutant background for various DEX or MOCK treatments. RNA was extracted from inflorescences at 0 and 4 hours after DEX or a MOCK treatment and used as probes for our microarray experiments. Three biological replicates of each were hybridized to Affymetrix ATH1 arrays. We used the Affymetrix Microarray Suite software (MAS 5.0) to identify genes whose expression profiles changed only after DEX-treatment and are likely targets of AP3/PI.
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| Contributor(s) |
Mara CD, Irish V |
| Citation(s) |
18417639 |
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| Submission date |
Nov 27, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Chloe Mara |
| Organization name |
Yale University
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| Department |
MCDB
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| Lab |
Vivian Irish
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| Street address |
160 Prospect Street
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA103607 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9702_RAW.tar |
62.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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