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| Status |
Public on Jun 21, 2018 |
| Title |
Capturing the onset of PRC2-mediated repressive domain formation (4C-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Our study defines nucleation and spreading regions for Polycomb repressive complex 2 (PRC2), demonstrating the principle of PRC2 domain formation in mammals. We elucidate the role of genome architecture in formation of these domains and identify JARID2 and MTF2 as being crucial for full recruitment of PRC2 to chromatin.
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| Overall design |
In this experiment, we performed 4C-seq in mESCs using nucleation sites, spreading sites, and two control sites as viewpoints.
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| Contributor(s) |
Oksuz O, Narendra V, Lee C, Descostes N, LeRoy G, Raviram R, Karch K, Rocha PR, Garcia BA, Skok JA, Reinberg D |
| Citation(s) |
29932905 |
| |
| Submission date |
Feb 02, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Danny Reinberg |
| E-mail(s) |
[email protected]
|
| Phone |
212-263-9036
|
| Organization name |
NYU School of Medicine
|
| Department |
Biochemistry and Molecular Pharmacology
|
| Lab |
Reinberg
|
| Street address |
522 First Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE94431 |
Capturing the onset of PRC2-mediated repressive domain formation |
|
| Relations |
| BioProject |
PRJNA369671 |
| SRA |
SRP098748 |
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