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| Status |
Public on Oct 24, 2007 |
| Title |
Gene expression in roots and shoots of plants grown on selenate |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Selenate is chemically similar to sulfate and can be taken up and assimilated by plants via the same transporters and enzymes. In contrast to many other organisms, selenium (Se) has not been shown to be essential for higher plants. In excess, Se is toxic and restricts development. Both Se deficiency and toxicity pose problems worldwide. To obtain better insight into the effects of Se on plant metabolism and into plant mechanisms involved in Se tolerance, the transcriptome of Arabidopsis plants grown with or without selenate was studied, and Se-responsive genes identified. Roots and shoots exhibited different Se-related changes in gene regulation and metabolism. Many genes involved in sulfur (S) uptake and assimilation were upregulated. Accordingly, Se treatment enhanced sulfate levels in plants, but the quantity of organic S metabolites decreased. Transcripts regulating the synthesis and signaling of ethylene and jasmonic acid were also upregulated by Se. Selenate appeared to repress plant development, as suggested by the down-regulation of genes involved in cell wall synthesis and auxin-regulated proteins. The Se-responsive genes discovered in this study may help create plants that can better tolerate and accumulate Se, which may enhance the effectiveness of Se phytoremediation or serve as Se-fortified food.
Keywords: selenate, abiotic stress
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| Overall design |
Arabidopsis thaliana (ecotype WS) plants were vertically grown on +/- 40 micromolar selenate MS medium. Root and shoot tissues of 10-day-old plants were seperated and used for RNA extraction and hybridization on Affymetrix microarrays. All conditions were duplicated.
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| Contributor(s) |
Van Hoewyk D, Takahashi H, Inoue E, Tamaoki M, Hess A, Pilon-Smits E |
| Citation(s) |
18251864 |
| |
| Submission date |
Oct 12, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Douglas Van Hoewyk |
| E-mail(s) |
[email protected]
|
| Phone |
970 472 5256
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| Fax |
970 472 5256
|
| Organization name |
Colorado State University
|
| Department |
Biology Department
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| Lab |
Pilon-Smits
|
| Street address |
1521 Crestmore PL
|
| City |
Fort Collins |
| State/province |
CO |
| ZIP/Postal code |
80521 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA102967 |
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