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| Status |
Public on Mar 07, 2019 |
| Title |
Genome-wide maps of chromatin state in neural stem/progenitor cells (NSC) from mouse neonatal forebrain [ChIA-PET] |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
We report the application of single-molecule-based DNA sequencing technology for high-throughput profiling of long-range chromatin interactions in Sox2-deleted and wild type NSC, by improved ChIA-PET procedure (in situ ChIA-PET) with antiRNApolII antibodies. By obtaining sequences from chromatin immunoprecipitated DNA, we generated genome-wide maps of inter-molecular ligated DNA representing long-range interactions in chromatin.
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| Overall design |
Analysis of RNApolII-mediated long-range interactions in chromatin of Sox2-deleted and wild type neural stem/progenitor cells (NSC) cultured from neonatal mouse brains.
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| Contributor(s) |
Wong C, Favaro R, Tjong H, Pagin M, Zhu Y |
| Citation(s) |
30849367 |
| |
| Submission date |
Nov 26, 2016 |
| Last update date |
May 21, 2019 |
| Contact name |
Federico Zambelli |
| Organization name |
University of Milan
|
| Department |
Department of Biosciences
|
| Street address |
Via Giovanni Celoria 26
|
| City |
Milano |
| ZIP/Postal code |
20133 |
| Country |
Italy |
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| Platforms (2) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (5)
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| GSM2406790 |
RNApolII ChIA-PET, Sox2-deleted cultured NSC |
| GSM3143597 |
RNApolII in situ ChIA-PET, Sox2-deleted cultured NSC (TR2) |
| GSM3143598 |
RNApolII in situ ChIA-PET, Sox2-deleted cultured NSC (TR3) |
| GSM3143599 |
RNApolII in situ ChIA-PET, Wild type cultured NSC (TR2) |
| GSM3143600 |
RNApolII in situ ChIA-PET, Wild type cultured NSC (TR3) |
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| This SubSeries is part of SuperSeries: |
| GSE90561 |
Sox2 is required for functional chromatin connectivity in brain-derived neural stem cells. |
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| Relations |
| BioProject |
PRJNA355015 |
| SRA |
SRP093983 |
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