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| Status |
Public on Jul 22, 2009 |
| Title |
Global expression profiling to study the effect of glyphosate herbicide treatment on Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Using whole genome microarray (Affymetrix ATH1) we studied the transcriptional response of Arabidopsis thaliana to glyphosate (Roundup Original) herbicde that inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme and thus disrupts aromaticamino acid biosynthesis. Few genes related to defense and secondary metabolism were altered.
Keywords: 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) inhibiting herbicide stress response
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| Overall design |
Surfactant (preference 0.25%) treated plants were used as carrier control group and EC50 concentration of glyphosate was used as the herbicide treatment group. Each of the control and treatment group consisted of 3 biological replicates and each biological replicates comprised leaves from 10 individual plants. RNA was extracted at 24h post treatment to study the transcriptional alterations caused by the herbicide treatment.
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| Contributor(s) |
Das M, Mader MT, Reichman JR, Watrud LS, Pfleeger TG, Schäffner AR, Olszyk DM |
| Citation(s) |
20043233 |
| |
| Submission date |
Aug 31, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Malay Das |
| E-mail(s) |
[email protected]
|
| Phone |
5417544315
|
| Fax |
5417544799
|
| Organization name |
National Research Council, US-EPA
|
| Department |
EEB
|
| Street address |
200 SW 35th STreet
|
| City |
Corvallis |
| State/province |
OR |
| ZIP/Postal code |
97333 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (6)
|
| GSM226328 |
Carrier biological rep1 (Glyphosate) |
| GSM226329 |
Carrier biological rep2 (Glyphosate) |
| GSM226330 |
Carrier biological rep3 (Glyphosate) |
|
| Relations |
| BioProject |
PRJNA102339 |
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