|
| Status |
Public on May 08, 2018 |
| Title |
Identification of PPARG regulated genes in human GM-CSF-primed monocyte-derived macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Identification of PPARG regulated genes in human GM-CSF polarized monocyte-derived macrophages by siRNA knock-down approaches.
|
| |
|
| Overall design |
Human peripheral blood monocytes from three independent healthy donors (1, 2 and 3) were isolated by anti-CD14-labeled magnetic microbeads. CD14+ monocytes were cultured for 7 days in RPMI 10% FCS containing GM-CSF (M1). After 7 days, M1 cells were transfected with 50 nM siRNA for PPARG (siPPARG) or siRNA with a scramble sequence (siControl) from Ambion, for 48 hours.Total RNA from each condition was extracted and purified using the RNA isolation kit (Macheray-Nagel). Labelled RNA was used as hybridization probes on human Agilent-072363 SurePrint G3 Human Gene Expression v3 8x60K (G4851C). All experimental procedures were performed following manufacturer instructions. Microarrays were scanned on an Agilent G2539A scanner. Scanned images and raw data were pre-processed with GeneSpring GX (Agilent).
|
| |
|
| Contributor(s) |
Corbí AL, Nieto Mazarrón C, Puig-Kröger A |
| Citation(s) |
29434585 |
| |
| Submission date |
Oct 14, 2016 |
| Last update date |
May 08, 2018 |
| Contact name |
Amaya Puig |
| E-mail(s) |
[email protected]
|
| Organization name |
Hospital Gregorio Marañón
|
| Department |
Immunology
|
| Lab |
Immunometabolism
|
| Street address |
Dr. Esquerdo 46
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28007 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
|
| Samples (6)
|
| GSM2345237 |
Macrophage GM-CSF + siControl replicate 1 |
| GSM2345238 |
Macrophage GM-CSF + siControl replicate 2 |
| GSM2345239 |
Macrophage GM-CSF + siControl replicate 3 |
| GSM2345240 |
Macrophage GM-CSF + siPPARg replicate 1 |
| GSM2345241 |
Macrophage GM-CSF + siPPARg replicate 2 |
| GSM2345242 |
Macrophage GM-CSF + siPPARg replicate 3 |
|
| Relations |
| BioProject |
PRJNA348527 |
Less...
More...