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| Status |
Public on Sep 14, 2017 |
| Title |
Newborn and young adult (2 months) mouse dorsal skin cell culture time-series RNA-Seq |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Organoid formation is critical to the progress of regenerative medicine. We use the formation of skin organoids from dissociated cells to study the self-organization process. Here we live-image the process which reveals an unexpectedly complex morphogenetic process: dissociated cells – aggregates – polarized cysts – fused cysts– planar skin – hair placodes. Transcriptome profiling and functional testing show orderly expression peaks of adhesion molecules, insulin-like growth factors, Wnts, extracellular matrix molecules, and matrix metalloproteinases are crucial to the sequential morphological transitions. We draw an analogy between these self-organizing morphogenetic behaviours and the self-assembly of molecules in biophysics, with different morphological stages connected by phase transition-like switches. Adult cells, normally stalled as aggregates, can be restored to reform hairy skin by reactivation of the self-organization process with timely supply of molecular cues. Physical processes and molecular events may work in tandem to achieve organoid self-formation in tissue engineering and in vivo.
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| |
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| Overall design |
Comparison of mRNA profiles in newborn and young adult (about 2 months) mouse dorsal skin reconstitution cells at different culture time points
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| |
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| Contributor(s) |
Chuong C |
| Citation(s) |
28798065, 37996466 |
| |
| Submission date |
Sep 15, 2016 |
| Last update date |
Dec 08, 2023 |
| Contact name |
Mingxing Lei |
| E-mail(s) |
[email protected]
|
| Phone |
3234422845
|
| Organization name |
University of Southern California
|
| Street address |
2011 Zonal Ave HMR 304A
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA343078 |
| SRA |
SRP089917 |
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