|
| Status |
Public on Nov 20, 2017 |
| Title |
Innate and adaptive lymphocytes sequentially shape the gut microbiota and lipid metabolism |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In support of our manuscript investigating the roles of ILCs and T cells in the maintenance of gut hoemostasis, we have performed RNAseq on terminal illeum of mice lacking either all adaptive immune cells (RAG1 -/-), deficient in T cells (TCRalpha -/-), or deficient in T cells but co-housed with wild-type mice and RAG1 -/- mice.
|
| |
|
| Overall design |
Tissues from three mice per group were analysed, and the following comparisions were made: RAG1-/- vs. WT C57BL/6 and TCRa-/- co-housed vs TCRa-/- seperately housed. Differential expression genes were identified at 1% FDR using DESeq2.
|
| |
|
| Contributor(s) |
Mao K, Martins AJ, Germain RN |
| Citation(s) |
29364878 |
| |
| Submission date |
Sep 09, 2016 |
| Last update date |
Sep 05, 2023 |
| Contact name |
Andrew Martins |
| E-mail(s) |
[email protected]
|
| Organization name |
Yale School of Medicine
|
| Department |
Immunobiology
|
| Street address |
100 College Street Rm 1155
|
| City |
New Haven |
| State/province |
Connecticut |
| ZIP/Postal code |
06510 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA342497 |
| SRA |
SRP087889 |
Less...
More...