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Series GSE85741 Query DataSets for GSE85741
Status Public on Apr 17, 2017
Title Paused RNA polymerase II inhibits new transcriptional initiation
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary RNA polymerase II (Pol II) pauses downstream of the transcription initiation site before beginning productive elongation. This pause is a key component of metazoan gene expression regulation. Some promoters have a strong disposition for Pol II pausing and often mediate faster, more synchronous changes in expression. This requires multiple rounds of transcription and thus cannot rely solely on pause release. But it is unclear how pausing affects the initiation of new transcripts during consecutive rounds of transcription. Using our recently developed ChIP-nexus method, we find that Pol II pausing inhibits new initiation. We propose that paused Pol II helps prevent new initiation between transcription bursts, which may reduce noise.
 
Overall design Refer to individual Series
 
Citation(s) 28504701
Submission date Aug 17, 2016
Last update date May 15, 2019
Contact name Julia Zeitlinger
E-mail(s) [email protected]
Organization name Stowers Institute for Medical Research
Lab Zeitlinger lab
Street address 1000 E 50th Street
City Kansas City
State/province MO
ZIP/Postal code 64110
Country USA
 
Platforms (2)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
GPL19132 Illumina NextSeq 500 (Drosophila melanogaster)
Samples (58)
GSM2283349 TAF2 ChIP-nexus (Control condtion) Rep1
GSM2283350 TAF2 ChIP-nexus (Control condtion) Rep2
GSM2283351 TBP ChIP-nexus (Control condtion) Rep1
This SuperSeries is composed of the following SubSeries:
GSE85738 Paused RNA polymerase II inhibits new transcriptional initiation [ChIP-nexus]
GSE85739 Paused RNA polymerase II inhibits new transcriptional initiation [ChIP-nexus spike-in]
GSE85740 Paused RNA polymerase II inhibits new transcriptional initiation [ChIP-Seq]
Relations
BioProject PRJNA339229

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85741_RAW.tar 409.0 Mb (http)(custom) TAR (of BW)
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