|
| Status |
Public on Aug 31, 2017 |
| Title |
Paf1 modulates Piwi/piRNA silencing capacity in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To test whether the RNA Polymerase II factor PAF1 affects PIWI silencing, we examined the transcriptome expression levels in Drosophila OSS cells following siRNA knockdown of these factors.
|
| |
|
| Overall design |
The total RNAs from OSS cells were extracted after siRNA knockdown of GFP negative control, PAF1, PIWI, and a double knockdown of both PAF1 and PIWI. The RNAs were then converted into strand-specific sequencing libraries with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina.
|
| |
|
| Contributor(s) |
Lau N, Clark J, Rahman R |
| Citation(s) |
28844648 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R00 HD057298 |
The Function of piRNAs and the piRNA Complex |
BRANDEIS UNIVERSITY |
NELSON C LAU |
| R01 AG052465 |
Transposon landscapes and transcriptome diversity in aging neurons |
BRANDEIS UNIVERSITY |
NELSON C LAU |
|
| |
| Submission date |
Jul 25, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
NELSON LAU |
| E-mail(s) |
[email protected]
|
| Organization name |
Boston University School of Medicine
|
| Department |
Biochemistry
|
| Street address |
72 E Concord St K219, Dept of Biochemistry
|
| City |
BOSTON |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA331099 |
| SRA |
SRP079656 |
Less...
More...