|
| Status |
Public on Jun 20, 2017 |
| Title |
Nascent RNA-seq in Kasumi-1 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Amino Enhancer of Split (AES) is essential for AML1-ETO induced self-renewal and leukemogenesis. To study the effect of AES on transcription regulation in AML1-ETO expressing Kasumi-1 cells, nascent transcripts in control (shctr) and AES knockdown (shAES) Kasumi-1 cells were labelled with uridine analogue 4-thioduridine with subsequent nascent RNA purification and next generation sequencing (Nascent RNA-seq).
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| |
|
| Overall design |
Examination of nascent transcription in Kasumi-1 cells under two conditions with duplicate.
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| |
|
| Contributor(s) |
Zhou F, Rohde C, Klein H, Müller-Tidow C |
| Citation(s) |
28650479 |
| |
| Submission date |
Apr 22, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Christian Rohde |
| E-mail(s) |
[email protected]
|
| Organization name |
Heidelberg University
|
| Lab |
Molecular Hematology and Oncology
|
| Street address |
Im Neuenheimer Feld 410
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE80602 |
AML1-ETO induces leukemia via C/D box snoRNA/RNPs. |
|
| Relations |
| BioProject |
PRJNA319361 |
| SRA |
SRP073721 |
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