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| Status |
Public on Jan 01, 2017 |
| Title |
Rhodobacter sphaeroides 2.4.1 pRKSorX144 versus pRK415 under singlet oxygen stress. |
| Organism |
Cereibacter sphaeroides 2.4.1 |
| Experiment type |
Expression profiling by array
|
| Summary |
To learn more about the function of SorX, we constructed an over-expression strain. For this purpose, the sorX gene together with its 28-bp upstream region was cloned into the over-expression vector pRK4352 and expressed in the R. sphaeroides wild-type 2.4.1 background. The corresponding strain consequently exhibits a SorX over-expression that is driven by the constitutive 16S rRNA (RSP_4352) promoter. The over-expression strain was compared to an empty vector control (pRK415). Total RNA from the over-expression (pRKSorX144) and from the empty vector control (pRK415) strain was extracted after 7 min of singlet oxygen stress and used for microarray analysis to investigate SorX-induced changes on transcriptome level.
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| Overall design |
RNA samples collected after 7 min of singlet oxygen stress were analyzed by two-color microarrays. Each microarray contains a pool of three independent biological replicates for each sample.
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| Contributor(s) |
Peng T, Berghoff BA, Klug G |
| Citation(s) |
27420112 |
| |
| Submission date |
Mar 21, 2016 |
| Last update date |
Apr 05, 2017 |
| Contact name |
Gabriele Klug |
| E-mail(s) |
[email protected]
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| Organization name |
Justus-Liebig University Gießen
|
| Department |
Molecular- and Microbiology
|
| Street address |
Heinrich-Buff-Ring 26-32
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| City |
Gießen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
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| Platforms (1) |
| GPL15457 |
Klug lab - Rhodobacter sphaeroides - 15k array Version 2 (144 sRNA; Agilent-027061) |
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| Samples (3) |
| GSM2095032 |
R. sphaeroides WT 2.4.1_pRKSorX144 versus pRK415 after 7 min of singlet oxygen stress_biological replicate 1 |
| GSM2095033 |
R. sphaeroides WT 2.4.1_pRKSorX144 versus pRK415 after 7 min of singlet oxygen stress_biological replicate 2 |
| GSM2095034 |
R. sphaeroides WT 2.4.1_pRKSorX144 versus pRK415 after 7 min of singlet oxygen stress_technical replicate 1 |
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| Relations |
| BioProject |
PRJNA315814 |
