|
| Status |
Public on May 13, 2016 |
| Title |
Sequencing Universal Human Reference RNA by Smart-seq and early barcoding library preparation methods |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Many library preparation methods are available for gene expression quantification. Here, we sequenced and analysed Universal Human Reference RNA (UHRR) prepared using Smart-Seq2, TruSeq (public data) and a protocol using unique molecular identifiers (UMIs) that all include the ERCC spike-in mRNAs to investigate the effects of amplification bias on expression quantification.
|
| |
|
| Overall design |
UHRR 10 and 12 replicates for Smart-seq2 and UMI-seq library preparation methods, respectively.
|
| |
|
| Contributor(s) |
Parekh S, Hellmann I |
| Citation(s) |
27156886 |
| |
| Submission date |
Dec 08, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Swati Parekh |
| Organization name |
Ludwig Maximillians University Munich
|
| Department |
Biology II
|
| Lab |
Anthropology & Human genetics
|
| Street address |
Grosshaderner Str. 2
|
| City |
Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA305438 |
| SRA |
SRP067154 |
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