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| Status |
Public on May 18, 2016 |
| Title |
Genome-wide analysis of long noncoding RNA (lncRNA) expression in colorectal cancer tissues from patients with liver metastasis |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
We performed a genome-wide analysis of lncRNA expression to identify novel targets for the further study of liver metastasis in CRC. Samples obtained from CRC patients were analyzed using Arraystar human 8×60K lncRNA/mRNA v3.0 microarrays chips to find differentially expressed lncRNAs and mRNAs; The results were confirmed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The differentially expressed lncRNAs and mRNAs were identified through fold-change filtering. Gene ontology (GO) and pathway analyses were performed using standard enrichment computational methods.
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| |
|
| Overall design |
Twelve primary CRC samples were obtained from six patients with CRC and liver metastasis (Exp G) and six patients with CRC without metastasis (Ctrl G).
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|
| Contributor(s) |
Chen D, Wang W |
| Citation(s) |
27165481 |
| |
| Submission date |
Nov 16, 2015 |
| Last update date |
Mar 12, 2018 |
| Contact name |
Dong Chen |
| E-mail(s) |
[email protected]
|
| Organization name |
First Affiliated Hospital, Zhejiang University
|
| Street address |
79# Qingchun Rd
|
| City |
Hangzhou |
| ZIP/Postal code |
310003 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL16956 |
Agilent-045997 Arraystar human lncRNA microarray V3 (Probe Name Version) |
|
| Samples (12)
|
| GSM1941515 |
descending colon cancer_with liver metastasis_original [Exp 1] |
| GSM1941516 |
descending colon cancer_with liver metastasis_original [Exp 2] |
| GSM1941517 |
rectal cancer_with liver metastasis_original [Exp 3] |
| GSM1941518 |
rectal cancer_with liver metastasis_original [Exp 4] |
| GSM1941519 |
ascending colon cancer_with liver metastasis_original [Exp 5] |
| GSM1941520 |
sigmoid colon cancer_with liver metastasis_original [Exp 6] |
| GSM1941521 |
descending colon cancer_no metastasis_original [Ctrl 1] |
| GSM1941522 |
ascending colon cancer_no metastasis_original [Ctrl 2] |
| GSM1941523 |
ascending colon cancer_no metastasis_original [Ctrl 3] |
| GSM1941524 |
descending colon cancer_no metastasis_original [Ctrl 4] |
| GSM1941525 |
ascending colon cancer_no metastasis_original [Ctrl 5] |
| GSM1941526 |
ascending colon cancer_no metastasis_original [Ctrl 6] |
|
| Relations |
| BioProject |
PRJNA302290 |
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