|
| Status |
Public on Dec 10, 2015 |
| Title |
DGCR8 acts as a novel adaptor for the exosome complex to degrade double-stranded structured RNAs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The Microprocessor complex (DGCR8/Drosha) is required for microRNA (miRNA) biogenesis but also binds and regulates the stability of several types of cellular RNAs. Of particular interest, DGCR8 controls the stability of mature small nucleolar RNA (snoRNA) transcripts independently of Drosha, suggesting the existence of alternative DGCR8 complex/es with other nucleases to process a variety of cellular RNAs. Here, we found that DGCR8 co-purifies with subunits of the nuclear exosome, preferentially associating with its hRRP6-containing nucleolar form. Importantly, we demonstrate that DGCR8 is essential for the recruitment of the exosome to snoRNAs and to human telomerase RNA. In addition, we show that the DGCR8/exosome complex controls the stability of the human telomerase RNA component (hTR/TERC). Altogether, these data suggests that DGCR8 acts as a novel adaptor to recruit the exosome complex to structured RNAs and induce their degradation.
|
| |
|
| Overall design |
[i] Examination of the RNA binding profile of hRRP6 (also known as EXOSC10) via iCLIP.
[ii] HeLa cells were transiently depleted of hRRP6 or DGCR8 using siRNAs. For a control an non-targetting (siNon) siRNA was used. Three biological replicates of each samples were sent for RNA sequencing.
|
| |
|
| Contributor(s) |
Macías S, Cordiner RA, Gautier P, Plass M, Cáceres JF |
| Citation(s) |
26687677 |
| |
| Submission date |
Sep 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Mireya Plass |
| E-mail(s) |
[email protected]
|
| Organization name |
MDC-Berlin
|
| Street address |
Robert-Rössle Str. 10
|
| City |
Copenhagen |
| ZIP/Postal code |
13092 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA296819 |
| SRA |
SRP064143 |
Less...
More...