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Series GSE73203

Query DataSets for GSE73203

Status

Public on Jun 08, 2016

Title

Convergent evolution of peptide-based quorum sensing required for virulence in a eukaryotic pathogen

Organism

Cryptococcus neoformans

Experiment type

Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing

Summary

Bacterial quorum sensing (QS) systems are characterized by the regulated biogenesis and sensing of specialized signaling molecules. Here, we describe a peptide, Qsp1, secreted by the fungal pathogen Cryptococcus neoformans. Mutants lacking Qsp1 are attenuated for infection, pulmonary accumulation, and growth within macrophages. Qsp1 promotes density-dependent cell wall integrity and resistance to extreme environments. Qsp1 is also required for a secreted aspartyl protease activity required for virulence. Further biochemical and large-scale genetic investigations reveal that cleavage of Qsp1 from the C-terminal of a pro-peptide requires a cell-associated serine protease and Qsp1 sensing requires a predicted oligopeptide importer. Strikingly, these features closely mirror the hallmarks of a peptide-based QS system found in gram-positive bacteria, despite no ancestral relationship between individual components. Our studies thus reveal a convergently evolved, peptide-based QS system required for the virulence of a fungal pathogen.

Overall design

RNA-Seq or ChIP-Seq to compare multiple Cryptococcus neoformans genotypes using the HiSeq 2500 platform

Contributor(s)

Homer CM, Summers DK, Goranov AI, Clarke SC, Wiesner DL, Diedrich JK, Moresco JJ, Toffaletti D, Upadhya R, Caradonna I, Petnic S, Pessino V, Cuomo CA, Lodge JK, Perfect J, Yates III JR, Nielsen K, Craik CS, Madhani HD

Citation(s)

Submission date

Sep 18, 2015

Last update date

May 15, 2019

Contact name

Christina M. Homer

E-mail(s)

[email protected]

Organization name

UCSF

Department

Biochemistry & Biophysics

Lab

Madhani Lab

Street address

600 16th St

City

San Francisco

State/province

CA

ZIP/Postal code

94143

Country

USA

Platforms (2)

GPL19081	Illumina HiSeq 2500 (Cryptococcus neoformans)
GPL21073	Illumina HiSeq 4000 (Cryptococcus neoformans)

Samples (47)

More...

GSM1888496	Gat201-FLAG ChIP-Seq Replicate 1
GSM1888497	Gat201-FLAG ChIP-Seq Replicate 2
GSM1888498	Gat201-FLAG ChIP-Seq Replicate 3

GSM1888499	Gat204-FLAG ChIP-Seq Replicate 1
GSM1888500	Gat204-FLAG ChIP-Seq Replicate 2
GSM1888501	Gat204-FLAG ChIP-Seq Replicate 3
GSM1888502	Liv3-FLAG ChIP-Seq Replicate 1
GSM1888503	Liv3-FLAG ChIP-Seq Replicate 2
GSM1888504	Liv3-FLAG ChIP-Seq Replicate 3
GSM1888505	Input ChIP-Seq Replicate 1
GSM1888506	Input ChIP-Seq Replicate 2
GSM1888507	Input ChIP-Seq Replicate 3
GSM1888508	Input ChIP-Seq Replicate 4
GSM1888509	CM18 WT RNA-Seq replicate 1
GSM1888510	CM18 WT RNA-Seq replicate 2
GSM1888511	gat201∆ RNA-Seq replicate 1
GSM1888512	gat201∆ RNA-Seq replicate 2
GSM1888513	gat204∆ RNA-Seq replicate 1
GSM1888514	gat204∆ RNA-Seq replicate 2
GSM1888515	liv3∆ RNA-Seq replicate 1
GSM1888516	liv3∆ RNA-Seq replicate 2
GSM1888517	Kn99alpha WT RNA-Seq replicate 1
GSM1888518	Kn99alpha WT RNA-Seq replicate 2
GSM1919140	Kn99alpha WT Log phase RNA-Seq replicate 1
GSM1919141	Kn99alpha WT Log phase RNA-Seq replicate 2
GSM1919142	Kn99alpha WT Log phase RNA-Seq replicate 3
GSM1919143	qsp1∆ Log phase RNA-Seq replicate 1
GSM1919144	qsp1∆ Log phase RNA-Seq replicate 2
GSM1919145	qsp1∆ Log phase RNA-Seq replicate 3
GSM1919146	Kn99alpha WT Saturated Experiment 1 RNA-Seq replicate 1
GSM1919147	Kn99alpha WT Saturated Experiment 1 RNA-Seq replicate 2
GSM1919148	qsp1∆ Saturated Experiment 1 RNA-Seq replicate 1
GSM1919149	qsp1∆ Saturated Experiment 1 RNA-Seq replicate 2
GSM1919150	qsp1∆ Saturated Experiment 1 RNA-Seq replicate 3
GSM1919151	Kn99alpha WT Saturated Experiment 2 RNA-Seq replicate 1
GSM1919152	Kn99alpha WT Saturated Experiment 2 RNA-Seq replicate 2
GSM1919153	Kn99alpha WT Saturated Experiment 2 RNA-Seq replicate 3
GSM1919154	qsp1∆ Saturated Experiment 2 RNA-Seq replicate 1
GSM1919155	qsp1∆ Saturated Experiment 2 RNA-Seq replicate 2
GSM1919156	qsp1∆ + peptide Saturated Experiment 2 RNA-Seq replicate 1
GSM1919157	qsp1∆ + peptide Saturated Experiment 2 RNA-Seq replicate 2
GSM1919158	qsp1∆liv3∆ Saturated Experiment 2 RNA-Seq replicate 1
GSM1919159	qsp1∆liv3∆ Saturated Experiment 2 RNA-Seq replicate 2
GSM1919160	qsp1∆liv3∆ Saturated Experiment 2 RNA-Seq replicate 3
GSM1919161	qsp1∆liv3∆ + peptide Saturated Experiment 2 RNA-Seq replicate 1
GSM1919162	qsp1∆liv3∆ + peptide Saturated Experiment 2 RNA-Seq replicate 2
GSM1919163	qsp1∆liv3∆ + peptide Saturated Experiment 2 RNA-Seq replicate 3

Relations

BioProject

SRA

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE73203_RAW.tar	3.3 Mb	(http)(custom)	TAR (of TXT)
SRA Run Selector
Raw data are available in SRA
Processed data provided as supplementary file

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