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Status |
Public on Jan 12, 2016 |
Title |
Microarray profiling of renal medulla / papilla and cortex during postnatal development |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
The aim of this study is to establish a comprehensive transcriptome atlas that enables identification of key molecular pathways and morphogenic events regulating postnatal renal medulla/papillary and cortex development. To achieve this, a microarray expression profiling was performed on postnatal day 0-90 renal medulla and cortex obtained from CD1 male mice.
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Overall design |
Renal medulla and cortex were regionally dissected from postnatal day 0-90 CD1 male mice, and total RNA extracted for microarray expression profiling. Each time point consists of RNA pooled from 4 biological replicates, and an Agilent Bioanalyser test was performed to assess RNA integrity prior to sample pooling. The microarray data was analysed with the use of lumi and limma packages (Bioconductor) in R.
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Contributor(s) |
Phua YL, Little MH |
Citation(s) |
26800422 |
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Submission date |
Aug 17, 2015 |
Last update date |
Feb 02, 2016 |
Contact name |
Dr. Yu Leng Phua |
Organization name |
Icahn School of Medicine at Mount Sinai
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Street address |
1 Gustave L. Levy Pl
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City |
New York City |
State/province |
New York |
ZIP/Postal code |
10029 |
Country |
USA |
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Platforms (1) |
GPL6105 |
Illumina mouse-6 v1.1 expression beadchip |
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Samples (18)
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Relations |
BioProject |
PRJNA293120 |