|
| Status |
Public on Jan 04, 2016 |
| Title |
MLX transcriptional regulation in muscle cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study set out to identify MLX transcriptional targets in muscle cells. C2C12 Myoblasts were virally transduced to increase MLX activity, by overexpression of the wild-type protein; and to decrease MLX activity by overexpression of a dominant negative MLX protein and by shRNA induced knockdown of MLX. Transcripts that were significantly and consistently regulated by the different modes of MLX modulation were identified. The largest proportion of these were genes encoding secreted proteins including growth factors, cytokines and extracellular proteins. We therefore conclude that MLX can regulate myokine transcripts.
|
| |
|
| Overall design |
mRNA profiles from C2C12 muscle cells with increased and decreased MLX activity were examined.
|
| |
|
| Contributor(s) |
Hunt LC, Finkelstein D, Demontis F |
| Citation(s) |
26584623 |
| |
| Submission date |
Jun 19, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
David Finkelstein |
| E-mail(s) |
[email protected]
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA287516 |
| SRA |
SRP059674 |
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