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| Status |
Public on Sep 30, 2016 |
| Title |
Gene expression profiling of generated sunitinib-resistant RCC cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
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| Summary |
The clinical response to sunitinib in advanced renal-cell carcinoma (RCC) is frequently limited in magnitude and duration due to drug resistance, but the underlying mechanisms remain elusive. To discover mechanisms of resistance, we developed drug-resistant cell lines and have their gene expressions profiled and compared. Results provide insight into the molecular mechanisms underlying sunitinib-resistance.
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| Overall design |
RCC cell lines 786-O and ACHN, which were initially sensitive to Erlotinib treatment, are injected subcutaneously into the flanks of nude mice. Mice were treated with sunitinib orally (40 mg/kg/day) in a standard 4 weeks on, 2 weeks off treatment schedule. After one cycle of treatment, RCC cells dissociated from xenografts were transplanted into secondary mice followed with the same sunitinib-treatment. RCC cells are finally obtained from the 3rd generation xenografts. 7Su3rd is a shortcut of 786-O cells with sunitinib-resistance from the 3rd generation. ACSu3rd is a shortcut of ACHN cells with sunitinib-resistance from the 3rd generation.Gene expression profiles of acquired resistant RCC cells 7Su3rd, ACSu3rd were compared to their parental counterpart 786-O and ACHN, respectively. We designed this experiment to screen for lncRNA which is required for sunitinib-resistance.
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| Contributor(s) |
Qu L, Chen C, Wang L, Wang H |
| Citation(s) |
27117758 |
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| Submission date |
Jun 03, 2015 |
| Last update date |
Sep 30, 2016 |
| Contact name |
le qu |
| E-mail(s) |
[email protected]
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| Organization name |
Second Military Medical University
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| Department |
Department of Urology
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| Street address |
440 North Chengdu Road
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| City |
Shanghai |
| ZIP/Postal code |
200003 |
| Country |
China |
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| Platforms (1) |
| GPL19748 |
Agilent-038314 CBC Homo sapiens lncRNA + mRNA microarray V2.0 (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA285805 |
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