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| Status |
Public on Feb 26, 2016 |
| Title |
Comprehensive Epigenomic Analysis Reveals Dynamic Regulatory Programs Of Blood Development (Dnase-Hypersensitivity) |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Embryonic hematopoiesis is regulated by the coordinated interaction between transcription factors and the epigenetic regulators driving developmental-stage specific gene expression but how this process drives hematopoietic specification and terminal differentiation is poorly understood. Here we generated RNA-Seq, DNase-Seq and ChIP-Seq data for histone marks and transcription factors from ES-cell derived purified cells representing six sequential stages of blood cell specification and differentiation. Our data reveal the binding patterns of specific transcription factors involved in the priming and maintenance of distal elements and inform how binding impacts on promoter activity. Functional studies based on these data uncovered a previously unrecognised role for Hippo signalling in mammalian hematopoietic specification. Finally, we present a dynamic core regulatory network model for hematopoiesis and demonstrate its utility for the design of reprogramming experiments. Our study represents a powerful resource for studying hematopoiesis and demonstrates how such data can advance our understanding of mammalian development.
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| Overall design |
ChIP-seq data of histone modifications and transcription factors, and RNA-seq data obtained from purified cells representing five sequential stages of murine blood cell specification and differentiation
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| Contributor(s) |
Goode DK, Obier N, Vijayabaskar MS, Lichtinger M, Lie-A-Ling M, Hannah R, Lilly AJ, Florowska M, Batta K, Challinor M, Wallace K, Assi SA, Cauchy P, Gilmour J, Hoogenkamp M, Westhead DR, Lacaud G, Kouskoff V, Göttgens B, Bonifer C |
| Citation(s) |
26923725, 29530939 |
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| Submission date |
May 20, 2015 |
| Last update date |
Jul 29, 2019 |
| Contact name |
MS Vijayabaskar |
| E-mail(s) |
[email protected]
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| Organization name |
University of Leeds
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| Department |
School of Molecular and Cellular Biology
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| Street address |
Woodhouse Lane
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| City |
Leeds |
| ZIP/Postal code |
LS2 9JT |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE69101 |
Comprehensive Epigenomic Analysis Reveals Dynamic Regulatory Programs Of Blood Development |
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| Relations |
| BioProject |
PRJNA284525 |
| SRA |
SRP058539 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE69095_RAW.tar |
1.0 Gb |
(http)(custom) |
TAR (of BED, BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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