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| Status |
Public on Feb 09, 2017 |
| Title |
Identification of candidate adherent-invasive E. coli signature transcripts by genomic/transcriptomic analysis |
| Organisms |
Escherichia coli HS; Escherichia coli LF82 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Our aim was to identify candidate transcripts that distinguish AIEC from non-invasive E. coli (NIEC) strains and might be useful for rapid and accurate identification of AIEC by culture-independent technology. We performed comparative RNA-Sequence (RNASeq) analysis using AIEC strain LF82 and NIEC strain HS during exponential and stationary growth.
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| Overall design |
Triplicate Luria broth cultures (37o C) of LF82 and HS were grown with continuous shaking for 2 hours (exponential phase) and 24 h without shaking (stationary phase).
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| Contributor(s) |
Gathungu G, Zhang Y, Rowehl L, Orner EP, Boedeker EC, Xiong X, Parkinson o, Li E |
| Citation(s) |
26125937 |
| |
| Submission date |
May 19, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Yuanhao Zhang |
| E-mail(s) |
[email protected]
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| Organization name |
Stony Brook University
|
| Department |
Applied Mathematics and Statistics
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| Street address |
AMS Department,Stony Brook University
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| City |
Stony Brook |
| ZIP/Postal code |
11790 |
| Country |
USA |
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| Platforms (2) |
| GPL20214 |
Illumina HiSeq 2500 (Escherichia coli HS) |
| GPL20215 |
Illumina HiSeq 2500 (Escherichia coli LF82) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA284327 |
| SRA |
SRP058478 |
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