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| Status |
Public on Feb 10, 2017 |
| Title |
Generation of PDGFRα+ Cardioblasts from Pluripotent Stem Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We uncover a novel class of cardiac lineage cells, PDGFRα+Flk1- cardioblasts (PCB), from mouse and human pluripotent stem cells (PSC) using CsAYTE, a combination of small molecules – Cyclosporin A (CsA), ROCK inhibitor Y27362, antioxidant Trolox, and ALK5 inhibitor EW7197.
To elucidate the genome-wide characteristics of PCB, microarray analysis was performed and compared with the spontaneously formed PCB, which were obtained without CsAYTE incubation (hereafter referred to as “sfPCB”), Flk1+ mesodermal precursor cells (MPC), and αMHC+ cardiomyocytes (M+CM)
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| Overall design |
Sorting of cells was performed by flow cytometry (Flk1+ MPC at day 4.5, sfPCB and PCB at day 5.5, and M+CM at day 10.5 from PSC differentiation) and microarray analysis was performed.
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| Contributor(s) |
Cho SW, Koh GY |
| Citation(s) |
28165490 |
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| Submission date |
Feb 10, 2015 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Gou Young Koh |
| E-mail(s) |
[email protected]
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| Phone |
82-42-350-2638
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| Organization name |
KAIST
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| Street address |
291 Daehark-ro, Yuseong-gu
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| City |
Daejeon |
| ZIP/Postal code |
305-701 |
| Country |
South Korea |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA275062 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE65791_RAW.tar |
8.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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