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Series GSE65660 Query DataSets for GSE65660
Status Public on Sep 18, 2015
Title TCF1 is required for the differentiation of T follicular helper (TFH) cells during viral infections
Organism Mus musculus
Experiment type Expression profiling by array
Summary TFH and Th1 cells generated after viral or intracellular bacterial infections are critical for the control of infections and the development of immunological memories. However, the mechanisms that govern the choice of activated CD4 T cells to the two alternative fates remain unclear. Here, we found that reciprocal expression of TCF1 and Blimp1 between viral-specific TFH and Th1 cells started early after infection. TCF1 was intrinsically required for the differentiation of TFH cells. In the absence of TCF1, TFH cells failed to maintain their transcriptional and metabolic signatures, distinct from those in Th1 cells. Mechanistically, TCF1 functioned through forming negative feedback loops with IL-2 and Blimp1 signaling. Thus, we have demonstrated an essential role of TCF1 in TFH-cell differentiation.
 
Overall design Tcf7 deficient and WT SMARTA CD4 T cells were isolated from mice 8 days after Lymphocytic Choriomeningitis Virus (LCMV) infection. TFH and Th1 cells were separated by FACS.
 
Contributor(s) Elkahloun AG, Wu T
Citation(s) 26365183
Submission date Feb 05, 2015
Last update date Feb 21, 2018
Contact name abdel G Elkahloun
E-mail(s) [email protected]
Phone 301 402 3170
Organization name NHGRI-NIH
Lab MICROARRAY CORE
Street address 50, SOUTH DRIVE
City BETHESDA
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL16570 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]
Samples (12)
GSM1602616 TW13_D8 KO TFH_1
GSM1602617 TW14_D8 KO Th1_1
GSM1602618 TW15_D8 WT TFH_1
Relations
BioProject PRJNA274631

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65660_RAW.tar 120.3 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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