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| Status |
Public on Jan 01, 2017 |
| Title |
mRNA expression in white adipose tissue of adult F2 female offspring from F0-fathers fed a chow or high-fat diet |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
The purpose of this study was to investigate whether paternal high-fat diet (HFD) transgenerationally remodels the epigenome of spermatozoa to alter metabolism in the F1 and F2 generation offspring
White adipose tissue mRNA expression profiling of F2-female offspring from F0-founders fed either a chow or a chronic HFD challenged. Adult females were challenged or not a high-fat diet for 12 weeks. White adipose tissue was dissected at an endpoint experiment. Rats were subjected to 4 hours fasting prior to anesthesia with pentobarbital and tissue collection.
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| Overall design |
F2-females Sprague-Dawley rats from F0 founders fed or not a high-fat diet were also subjeted to high-fat consumption for 12 weeks. At terminal experiment, gonadal white adipose from 6 to 7 animals per group was collected, prepared and subjeted to the array analysis.
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| Contributor(s) |
Barres R, Zierath J, Krook A, de Castro Barbosa T |
| Citation(s) |
26977389 |
| |
| Submission date |
Jan 22, 2015 |
| Last update date |
Mar 14, 2017 |
| Contact name |
Thais de Castro Barbosa |
| Organization name |
Karolinska Institutet
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| Department |
Physiology and Pharmacology
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| Lab |
Integrative Physiology
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| Street address |
von Eulers väg 4a
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| City |
Stockholm |
| State/province |
Stockholm |
| ZIP/Postal code |
171 77 |
| Country |
Sweden |
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| Platforms (1) |
| GPL11534 |
[RaGene-1_1-st] Affymetrix Rat Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA273353 |
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