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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 23, 2015 |
| Title |
Role of IL-2 inducible T cell kinase (ITK) and Bruton's tyrosine kinase (BTK) in mast cell response to lipopolysaccharide (LPS) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mast cells are indispensable for LPS-induced septic hypothermia, in which TNF-α plays an essential role to initiate sepsis. Tec family non-receptor tyrosine kinases ITK and BTK regulate mast cell-derived TNF-α in response to allergic antigen, but their role in LPS-induced TNF-α production by mast cells and related pathology is unclear. We sought to investigate the role(s) of ITK and BTK in mast cell response in septic condition. We found that the absence of ITK and BTK leads to enhanced TNF-α production by bone marrow-derived mast cells (BMMC). Itk-/-Btk-/- mast cells exhibit hyperactive preformed and LPS-induced TNF-α production, along with enhanced expression of other related genes such as NF-κB targeted genes, compared to WT cells.
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| Overall design |
Bone marrow cells from 8-week old WT, Itk-/-, Btk-/- and Itk-/-Btk-/- (double knockout: DKO) C57Bl/6 mice were cultured in murine Interleukin-3/Stem cell factor (IL-3/SCF) supplemented medium for 5 weeks to derive mast cells. WT, Itk-/-, Btk-/- and DKO bone marrow-derived mast cells (BMMC) were factor starved in medium without IL-3/SCF for 12 hours, followed by treatment with PBS (control) or 100 ng/ml LPS for 1 hour. Triplicates of each group were subjected to mouse whole genome genechip microarray analysis. Replicates were randomized on different chips to avoid systematic error.
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| Contributor(s) |
Huang W, August A |
| Citation(s) |
26581914, 27081634 |
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Huang W, August A. Role(s) of IL-2 inducible T cell kinase and Bruton?s tyrosine kinase in mast cell response to lipopolysaccharide. Genomics Data. 2016;8:18-20. doi:10.1016/j.gdata.2016.02.010.
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| Submission date |
Dec 17, 2014 |
| Last update date |
May 29, 2020 |
| Contact name |
Weishan Huang |
| E-mail(s) |
[email protected]
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| Organization name |
Cornell University
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| Department |
Microbiology & Immunology
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| Lab |
Avery August
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| Street address |
C5 121, Cornell U.
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| City |
Ithaca |
| State/province |
New York |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (24)
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| GSM1568011 |
WT BMMC with PBS as control, biological rep1 |
| GSM1568012 |
WT BMMC with PBS as control, biological rep2 |
| GSM1568013 |
WT BMMC with PBS as control, biological rep3 |
| GSM1568014 |
Itk-/- BMMC with PBS as control, biological rep1 |
| GSM1568015 |
Itk-/- BMMC with PBS as control, biological rep2 |
| GSM1568016 |
Itk-/- BMMC with PBS as control, biological rep3 |
| GSM1568017 |
Btk-/- BMMC with PBS as control, biological rep1 |
| GSM1568018 |
Btk-/- BMMC with PBS as control, biological rep2 |
| GSM1568019 |
Btk-/- BMMC with PBS as control, biological rep3 |
| GSM1568020 |
DKO BMMC with PBS as control, biological rep1 |
| GSM1568021 |
DKO BMMC with PBS as control, biological rep2 |
| GSM1568022 |
DKO BMMC with PBS as control, biological rep3 |
| GSM1568023 |
WT BMMC with 100ng/ml LPS, biological rep1 |
| GSM1568024 |
WT BMMC with 100ng/ml LPS, biological rep2 |
| GSM1568025 |
WT BMMC with 100ng/ml LPS, biological rep3 |
| GSM1568026 |
Itk-/- BMMC with 100ng/ml LPS, biological rep1 |
| GSM1568027 |
Itk-/- BMMC with 100ng/ml LPS, biological rep2 |
| GSM1568028 |
Itk-/- BMMC with 100ng/ml LPS, biological rep3 |
| GSM1568029 |
Btk-/- BMMC with 100ng/ml LPS, biological rep1 |
| GSM1568030 |
Btk-/- BMMC with 100ng/ml LPS, biological rep2 |
| GSM1568031 |
Btk-/- BMMC with 100ng/ml LPS, biological rep3 |
| GSM1568032 |
DKO BMMC with 100ng/ml LPS, biological rep1 |
| GSM1568033 |
DKO BMMC with 100ng/ml LPS, biological rep2 |
| GSM1568034 |
DKO BMMC with 100ng/ml LPS, biological rep3 |
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| Relations |
| BioProject |
PRJNA270640 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE64287_Gene-level_analysis_quantile-normalized.txt.gz |
4.2 Mb |
(ftp)(http) |
TXT |
| GSE64287_Probe-level_analysis_with_annotation.txt.gz |
6.4 Mb |
(ftp)(http) |
TXT |
| GSE64287_RAW.tar |
53.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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