|
| Status |
Public on May 20, 2015 |
| Title |
Drop-Seq analysis of mixtures of human (HEK) and mouse (3T3) cells (1000/100 STAMPs) at 50 cells per microliter |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
A cell supsension containing an equal mix of HEK and 3T3 cells was used in Drop-Seq, a novel technology for high-throughput single cell mRNAseq. The cells were mixed at a final concentration of 50 cells per microliter.
|
| |
|
| Overall design |
An estimated 1000 STAMPs and 100 STAMPs were separately selected for sequencing to obtain both high coverage and broad coverage data
|
| |
|
| Contributor(s) |
Macosko EZ |
| Citation(s) |
26000488, 32888429 |
| |
| Submission date |
Nov 13, 2014 |
| Last update date |
Apr 25, 2022 |
| Contact name |
Evan Macosko |
| E-mail(s) |
[email protected]
|
| Organization name |
Harvard Medical School
|
| Department |
Genetics
|
| Lab |
McCarroll Lab
|
| Street address |
77 Avenue Louis Pasteur, NRB 260
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
|
| Samples (2) |
| GSM1544798 |
SpeciesMix_ThousandSTAMPs_50cellspermicroliter |
| GSM1544799 |
SpeciesMix_HundredSTAMPs_50cellpermicroliter |
|
| This SubSeries is part of SuperSeries: |
| GSE63473 |
Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets |
|
| Relations |
| BioProject |
PRJNA267124 |
| SRA |
SRP049775 |
