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| Status |
Public on Jul 26, 2015 |
| Title |
Gene expression profiling of human Ewing sarcoma cells after knockdown of EGR2 or EWSR1-FLI1. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To get insight in the functional role of EGR2 for Ewing sarcoma, we performed a transcriptional profiling of Ewing sarcoma cells after knockdown of EGR2 and compared the resulting transcriptional signature with that of EWSR1-FLI1-silenced Ewing sarcoma cells. In accordance with the strong EGR2-induction by EWSR1-FLI1, both genes highly significantly overlap in their transcriptional signatures. Gene-set enrichment analyses (GSEA) and DAVID (Database for Annotation, Visualisation and Integrated Discovery) gene ontology analyses indicated a strong impact of EGR2 on cholesterol and lipid biosynthesis resembling its function in orchestrating lipid metabolism of myelinating Schwann cells.
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| Overall design |
A673 and SK-N-MC Ewing sarcoma cells were transfected with specific siRNAs directed against EGR2 or EWSR1-FLI1 or non-targeting control siRNA. 48 h thereafter RNA was harvested and processed for microarray analysis.
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| Contributor(s) |
Grunewald TG, Delattre O |
| Citation(s) |
26214589, 35565457 |
| |
| Submission date |
Oct 06, 2014 |
| Last update date |
May 25, 2022 |
| Contact name |
Thomas Georg Philipp Grunewald |
| E-mail(s) |
[email protected]
|
| Phone |
0049-6221-423718
|
| Organization name |
German Cancer Research Center (DKFZ)
|
| Department |
Division of Translational Pediatric Sarcoma Research
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL19264 |
[HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array [HuGene21st_Hs_ENTREZG_16.0.0] |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA263177 |
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