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| Status |
Public on Sep 25, 2015 |
| Title |
Nanos2-regulated mRNAs in the cultured spermatogonial stem cells (SSCs) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Spermatogonial stem cells are foundation of spermatogenesis. In our previous study, we observed a rapid degeneration of spermatogenesis by conditional knockout (cKO) of Nanos2 in adult spermatogonia, but the underlying mechanisms are largely unknown. While, conditional overexpression (cOE) of Nanos2 also inhibited germ cell development and stayed in undifferentiation status. Here we used cultured germline stem cells (GSCs) to study the mechanisms of Nanos2 in spermatogonia. We compared Nanos2 expression in cultured GSCs with in vivo isolated Nanos2 positive cells, and found the level of Nanos2 is significantly lower in GSCs as compared to Nanos2 positive cells. Thus we generated Nano2 cOE GSCs lines. By conditional inducing expression of Nanos2 we compared gene expression profile to control GSCs. These data provide the first evidence of Nanos2 regulating genes in spermatogonial stem cells.
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| Overall design |
Ectopic induction of Nanos2 in cultured GSCs. Duplicated control (Roa-CreERT2;CAG-CAT-3FlagNanos2-pA, DMSO) and experiment (Roa-CreERT2;CAG-CAT-3FlagNanos2-pA, Tamoxifen)
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| Contributor(s) |
Zhou Z, Yumiko S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 26, 2014 |
| Last update date |
Jan 12, 2017 |
| Contact name |
ZHI ZHOU |
| E-mail(s) |
[email protected]
|
| Phone |
055-981-6832
|
| Organization name |
National Institute of Genetics, Japan
|
| Department |
Division of Mammalian Development
|
| Lab |
Mammalian Development laboratory
|
| Street address |
Yata 1111
|
| City |
Mishima |
| ZIP/Postal code |
4118540 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
|
| Samples (4)
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| GSM1514380 |
Nanos2_conditional_overexpression_Germline_stem_cells_treated_with_DMSO_1 |
| GSM1514381 |
Nanos2_conditional_overexpression_Germline_stem_cells_treated_with_DMSO_2 |
| GSM1514382 |
Nanos2_conditional_overexpression_Germline_stem_cells_treated_with_4-Tamoxifen_1 |
| GSM1514383 |
Nanos2_conditional_overexpression_Germline_stem_cells_treated_with_4-Tamoxifen_2 |
|
| Relations |
| BioProject |
PRJNA262174 |
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